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Effects of exogenous hydrogen sulfide on brain metabolism and early neurological function in rabbits after cardiac arrest

Xia Wei| Le Duan| Liqun Bai| Miaomiao Tian| Wenzhi Li| Bing Zhang
Experimental
Volume 38, Issue 11 / November , 2012

Pages 1877 - 1885

Abstract

Purpose

Some of the neuroprotective effects of hydrogen sulfide (H2S) have been attributed to systemic hypometabolism and hypothermia. However, systemic metabolism may vary more dramatically than brain metabolism after cardiac arrest (CA). The authors investigated the effects of inhaled exogenous hydrogen sulfide on brain metabolism and neurological function in rabbits after CA and resuscitation.

Methods

Anesthetized rabbits were randomized into a sham group, a sham/H2S group, a CA group, and a CA/H2S group. Exogenous 80 ppm H2S was administered to the sham/H2S group and the CA/H2S group which suffered 3 min of untreated CA by asphyxia and resuscitation. Effects on brain metabolism (cerebral extraction of oxygen (CEO2), arterio-jugular venous difference of glucose [AJVD(glu)] and lactate clearance), S100B, viable neuron counts, neurological dysfunction score, and survival rate were evaluated.

Results

CEO2, AJVD(glu), and lactate increased significantly after CA. Inhalation of 80 ppm H2S significantly increased CEO2 (25.04 ± 7.11 vs. 16.72 ± 6.12 %) and decreased AJVD(glu) (0.77 ± 0.29 vs. 1.18 ± 0.38 mmol/L) and lactate (5.11 ± 0.43 vs. 6.01 ± 0.64 mmol/L) at 30 min after resuscitation when compared with the CA group (all P < 0.05). In addition, neurologic deficit scores, viable neuron counts, and survival rate were significantly better whereas S100B was decreased after H2S inhalation.

Conclusions

The present study reveals that inhalation of 80 ppm H2S reduced neurohistopathological damage and improves early neurological function after CA and resuscitation in rabbits. The increased CEO2 and decreased AJVD(glu) and enhanced lactate clearance may be involved in the protective effects.

Keywords

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